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KMID : 0362820110300020104
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Journal of the Korean Academy of Implant Dentistry
2011 Volume.30 No. 2 p.104 ~ p.114
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Evaluation of periodontal disease related bacteria from the implant-abutment interface
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Jung Da-Un
Sim Hye-Young
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Abstract
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Purpose: The purpose of this study was to investigate the detection rate of putative periodontopathogens, Porphyromonas. gingivalitis, Prevotella. intermedius, Tannerella. forsythensis, Treponema. denticola, Actinobacillus. actionmycetemcomitans, related to implant-abutment interface microleakage.
Materials and Methods: Samples were taken from 27 subjects with sterilized paper points and were transported in 1*PBS. The detection of periodontopathogens were performed by polymerase chain reaction with species-specific primers based on 16S rDNA. The PCR products were cloned into pGEM-T easy vector and its nucleotides were sequenced in order to confirm the specificity.
Results: Our data showed that the detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in implant fixture was 59% and 82% in patients respectively. Detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in implant crevice was 44% and 82% in patients. Detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in tongue was 82% and 82% in patients.
Conclusion: Current implant systems cannot safely prevent microbial leakage and bacterial colonization of the inner part of the implant.
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KEYWORD
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Implant-abutment interface, Periodontopathogens, Microbial leakage, Porphyromonas gingivalisvalis
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